The Next Gen DNA Library Kit is designed to prepare Next generation sequencing (NGS) libraries from double-stranded input DNA for use with Illumina® sequencing platforms. Libraries can be generated from as little as 10 pg of high-quality genomic DNA, ChIP DNA, FFPE DNA or cell-free DNA (cfDNA), which is 500-fold more sensitive than other library preparation kits. Additionally, libraries can be prepared from 100 ng DNA if preparing PCR-free libraries.

Next Gen DNA Library Kit is used in combination with the Next Gen Indexing Kit, which contains 16 unique index adapters that may be used individually, or in combination to multiplex samples together during cluster generation for co-sequencing on the same Illumina flow cell. The advantage of the Next Gen DNA Library preparation is the inclusion of molecular identifiers (MIDs) to enable accurate removal of PCR duplicates from single read sequencing. This helps to increase the number of unique alignments for more accurate data sets.

Features

Next Gen DNA Library Kit Highlights

• NGS libraries for use with Illumina platforms (MiSeq, HiSeq 2500, NextSeq 500)
• Works with double-stranded input DNA including: genomic DNA, ChIP DNA, FFPE DNA and cell-free DNA (cfDNA)
• Use as little as 10 pg DNA (500-fold less input material required than other kits)
• Prepare PCR-free libraries with only 100 ng DNA
• Indexing kit contains 16 unique indices that can be multiplexed together within the same sequencing flow cell
• Compatible with Active Motif’s ChIP-IT High Sensitivity® Kit for a complete ChIP-seq workflow