The Nuclear Extract Kit isolates high-quality nuclear, cytoplasmic or whole-cell extract from mammalian cell or tissue samples. This eliminates the need for optimizing reagents and protocols, and ensures high yields every time. More importantly, standardizing your extraction procedure with our high-quality reagents ensures more reproducible results. Extracts are suitable for use in a various applications including EMSA, Western blot and binding assays and is ideally suited for preparation of extracts for our TransAM® Transcription Factor Assays.

The Nuclear Extract Kit works with fresh or frozen cell and tissue samples and yields protein amounts of approximately 0.5 to 1 mg from cytoplasmic extract (at 1-2 mg/ml concentration), 150 to 250 μg from nuclear extract (at 3-5 mg/ml concentration), 1.2 to 2.4 mg from whole cell extract (at 4-8 mg/ml concentration) from 8.8 x 106 cells. The kit procedure is simple and only takes two hours to complete. The kit includes buffers, detergents and reagents for lysis as well as Protease and Phosphatase inhibitors to ensure preservation of your proteins.

Applications
Extracts prepared using the Nuclear Extract Kit can be used with Active Motif’s TransAM® Kits, or in other procedures that require cell extracts, like gelshift assays, Western blots, DNA footprinting, as a starting point for protein purification for binding or activity assays or or other analysis techniques.

Features

Nuclear Extract Kit advantages

• Quality-controlled reagents ensure reproducibility
• No need to optimize your own procedure
• Complete kit contains all required reagents
• Prepare nuclear, cytoplasmic or whole-cell extracts with one kit

Nuclear Extract Kit Contents

Contents & Storage
The Nuclear Extraction Kit is shipped on dry ice and contains reagents with multiple storage temperatures inside. Please store each component at the temperature indicated below. All reagents are guaranteed stable for 6 months from date of receipt when stored properly.

• 1 M DTT (Dithiothreitol); Store at -20°C
• Protease Inhibitor Cocktail (PIC); Store at -20°C
• Lysis Buffer AM1; Store at 4°C
• 10X PBS; Store at 4°C
• Phosphatase Inhibitors; Store at 4°C
• 10X Hypotonic Buffer; Store at 4°C
• Detergent; Store at 4°C