Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. Active Motif’s ChIP-IT® Express Kits eliminate some of these challenges by providing a streamlined protocol using protein G-coated magnetic beads, making it possible to perform ChIP in just 1 day. Kits that use either sonication or enzymatic digestion for preparing sheared chromatin are available. Complete your experimental design with Active Motif’s ChIP-IT® Control Kits, qPCR Primer Sets and Ready-to-ChIP Chromatin to validate your results at every step. To see how these features compare to the other ChIP Kits we offer, see our ChIP Kit Selection Guide.
How does it work?
ChIP involves the immunoprecipitation of protein/DNA complexes that have been stabilized via cross-linking. First, intact cells are fixed using formaldehyde, which cross-links and therefore preserves protein/DNA interactions. DNA is then sheared into small uniform fragments and the DNA/protein complexes are immunoprecipitated using an antibody directed against the DNA-binding protein of interest. Following immunoprecipitation, the DNA is washed, cross-linking is reversed, and the proteins are removed by Proteinase K treatment. Eluted DNA can be purified with Active Motif’s Chromatin IP DNA Purification Kit and is ready for downstream analysis via qPCR or Next-generation sequencing.
