The High Throughput ChIP-IT® Kit provides an optimized protocol and all the reagents needed to perform ChIP assays for histone modifications for transcription factors from 24 cell or tissue samples, yielding qPCR- or next-generation sequencing-ready samples with much less hands-on time than traditional methods.

This kit is designed to work with chromatin sheared with the PIXUL™ Multi-Sample Sonicator to give a robust, streamlined workflow compatible with processing multiple samples as efficiently as possible.

The kit works with chromatin samples prepared by other methods as well. Simply dilute your samples with the 5X ChIP Buffer provided in the kit and they are ready for High Throughput ChIP-IT® processing.

Features

High Throughput ChIP-IT® Kit Advantages

• Optimized protocol and reagents ensure efficient results
• Streamlined workflow reduces both total time and hands-on time relative to traditional ChIP protocols
• Low chromatin input required, 70 ng (10,000 cell equivalents) for histone ChIP, 333 ng (50,000 cells) for transcription factor ChIP
• Optimized reverse-crosslinking step in only 2 hours